FIG. 4.
L.A. repression of hypoxia-induced complex formation in LORE. EMSAs were performed as described in Materials and Methods. A 10-μg sample of CE from S. cerevisiae grown under normoxic (N) or hypoxic (H) conditions was incubated for 20 min at 25°C with a 32P-labeled LORE and then subjected to electrophoresis at 4°C for ∼3 h. Free probe (F) and bound complexes (B1 and B2) were detected by autoradiography. A minus sign indicates that no CE was added or that the CE was from cells grown without L.A. A plus sign indicates that CE was added. The letter P represents the DNA probe. CEs from yeast cells grown in increasing concentrations of L.A. (10, 25, and 100 μM) were used as indicated.