FIG. 6.

Activities of truncated Mga2p constructs under different conditions. The truncated constructs are shown on the left. The narrow line represents the Mga2p amino acid sequence. The numbers above each narrow line indicate positions in the amino acid sequence with respect to the N terminus on the left and the C terminus on the right. WT indicates that reporter (pAM6) analyses were performed with wild-type strain BY4741. Δmga2+MGA2 indicates that reporter (pAM6) analyses were performed with the BY4741Δmga2 strain transformed with pAM23 containing full-length MGA2. Δmga2+MGA2Δ172 indicates that strain BY4741Δmga2 transformed with pAM31 was used. Δmga2+MGA2Δ244 indicates that strain BY4741Δmga2 transformed with pAM30 was used. L.A indicates growth medium containing 100 μM L.A. dissolved in 1% ethanol. A plus sign indicates that L.A. was added; a minus sign indicates that no L.A. was added. The diagram on the right shows β-galactosidase activities under normoxic (N) and hypoxic (H) conditions. The absolute units of activity indicated are averages of at least three independent experiments that were performed in quadruplicate.