TABLE 1.
S. cerevisiae strains and plasmids used in this study
| Strain or plasmid | Genotype or plasmid description | Source or reference |
|---|---|---|
| RZ53-6 | MATatrp1-289 leu2-3, 112 ura3-52 ade1-100 | R. Zitomer |
| BY4741 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | RGa |
| BY4741Δmga2 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ΔMGA2 | RG |
| p62::934 | OLE1 promoter-lacZ fusion | 5 |
| p62::396 | OLE1 promoter-lacZ fusion | 5 |
| pAM4 | LORE in OLE1 promoter contains mutations in p62::934 | 38 |
| pTBA30 | Basal CYC1 promoter-lacZ fusion | A. K. Vershon |
| pAM6 | Tandem repeat LORE-basal CYC1 promoter-lacZ fusion | 38 |
| pAM23 | Centromeric plasmid; contains 5.1-kb fragment that contains MGA2 | 19 |
| pAM30 | Derivative of pAM23 with deletion of 244 aab in MGA2 C terminus | This study |
| pAM31 | Derivative of pAM23 with deletion of 172 aa in MGA2 C terminus | This study |
| pRS315 | Parental centromeric plasmid of pAM23 | ATCCc |
| YEplac181-mycMGA2 | 2μm plasmid; contains triple-myc-tagged MGA2 | 14 |