TABLE 2.
Accuracy of the data seta
| GSS ID (query) | nt identity (%)b | GenBank ID (target) | Gene | Stock |
|---|---|---|---|---|
| PT005A23R | 475/475 (100) | PTALPHA51 | Surface antigen | 51 |
| PT007O13R | 472/472 (100) | PTU19464 | Dynein beta heavy chain | 51 |
| PT009I01U | 323/323 (100) | PTE272425 | Eta-tubulin | d4-2 |
| PT011E03U | 621/624 (99) | AF149979 | X gene in rRNA spacer | 51 |
| PT012E03R | 366/367 (99.7) | PTSSRRN | 18S rRNA gene | 51 |
| PT021E13U | 490/500 (98) | PTY17649 | Calcium ATPase | 51 |
| PT012A14U | 485/486 (99.8) | AF149979 | 26S rRNA gene | 51 |
| PT014M07R | 453/453 (100) | PTU47117 | TMP4a secretory protein | d4-2 |
| PT014O15R | 150/152 (98.7) | PTG1IA1 | Surface antigen | 51 |
a
The database identification (ID) of the nine GSS sequences with BLASTN matches to previously characterized Paramecium genes are given with statistics concerning the matches. Examination of the chromatograms revealed a small dye peak in PT021E13U that accounts for the discrepancy with sequence PTY17649. No other obvious errors in base calling were found and, for at least some of the four other matches presenting with <100% identity, the differences may be due to genetic polymorphism since we have sequenced strain d4-2 derived from stock 51 and stock 29, whereas some Paramecium labs work with stock 51 (54).
b
That is, the number of matching nucleotides/total number of nucleotides.