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. 2002 Jun;1(3):440–447. doi: 10.1128/EC.1.3.440-447.2002

FIG. 2.

FIG. 2.

Northern blot analysis of gasA expression. Total RNA from the P. marneffei wild-type strain (FRR2161) was isolated from yeast cultures (37°C), vegetative mycelia grown in liquid at 25°C for 2 days (25°C veg), and asexually developing cultures (25°C dev), as indicated in Material and Methods. RNA from each of the three growth stages (37°C, 25°C veg, and 25°C dev) was hybridized with probes specific for either gasA or histone H3 (H3), which was used as a loading control. Arrows indicate the two gasA transcripts of 1.1 and 1.3 kb.