FIG. 5.

Analysis of the gasA^G42R, gasA^G203R, and ΔgasA strains at 37°C. (A) Colonial morphologies of the P. marneffei wild-type (FRR2161), dominant activated gasA^G42R, dominant interfering gasA^G203R, and ΔgasA strains. The strains were grown on SD medium at 37°C for 4 days. All strains exhibited normal yeast-like colonies except for the ΔgasA strain, which showed smaller colonies. This phenotype was found not to result from the inactivation of gasA (see the text). (B) Microscopic examination of the P. marneffei wild-type (FRR2161), dominant activated gasA^G42R, dominant interfering gasA^G203R, and ΔgasA strains. The strains were grown on BHI broth at 37°C for 4 days. All strains exhibited wild-type yeast cells. Scale bars, 20 μm. Differential interference contrast (DIC) and DAPI-stained epifluorescence of nuclei (DAPI) are shown. (C) Northern blot analysis of the P. marneffei wild-type (FRR2161), dominant activating gasA^G42R, dominant interfering gasA^G203R, and ΔgasA strains. Total RNA was isolated from yeast cultures grown in liquid at 37°C for 6 days. Northern blots were probed with either brlA, abaA, or histone H3 (H3). WT, wild type.