FIG. 2.
(A) Membrane localization required Cdc42p CTIL and polylysine residues. Plasmid p415MET(GFP-cdc42C188S) was transformed into wild-type strain TRY11-7D, and plasmids p415MET(GFP-CTIL) and p415MET(GFP-KKSKKCTIL) were transformed into wild-type strain W303. Transformants were grown to mid-log phase in SC−Leu−Met medium. (B) Constitutively active Cdc42G12Vp showed aberrant clustering. Plasmid p415MET(GFP-cdc42G12V) was transformed into wild-type TRY11-7D cells, and transformants were grown to mid-log phase in SC−Leu+Met medium. Images were assembled as in Fig. 1.