FIG. 5.

(A) Cdc42p localization in myo1Δ mutant. p415MET(GFP-CDC42) was transformed into myo1Δ strain YEF2125, and transformants were grown to mid-log phase in SC−Leu−Met medium. Images were assembled as in Fig. 1. (B) Time-lapse microscopy of GFP-Cdc42p clustering at the mother-bud neck region in wild-type and bni1Δ cells. p415MET(GFP-CDC42) was transformed into wild-type (TRY11-7D) and bni1Δ (YEF2126) cells, and transformants were grown to mid-log phase in SC−Leu−Met medium and observed as in Fig. 3. Fluorescence pictures were captured at ≈1- to 2-min intervals, with time zero representing the time at which GFP-Cdc42p was first observed at the mother-bud neck region. The cells shown are representative of at least 18 wild-type (upper panels) and 6 bni1Δ (lower panels) cells documented.