FIG. 6.

Cdc42p localization in septin cdc12-6^ts mutant. Plasmid p415MET(GFP-CDC42) was transformed into septin mutant cdc12-6^ts cells (CSO-1B), and transformants were grown to early to mid-log phase in SC−Leu−Met medium at 23°C. Half of the culture was then shifted to the restrictive temperature of 37°C. Both 23°C and 37°C cultures were observed for fluorescence. Upper left panel shows transformants grown at 23°C; upper right, lower left, and lower right panels show transformants shifted to 37°C for 1 h, 3 h, and 6.5 h, respectively. (B) Transformed cdc12-6 cells from A were shifted to the restrictive temperature of 37°C for 2.5 h, shifted back to the permissive temperature of 23°C for 1.5 h, and then shifted back to 37°C for 1 h. (C) Colocalization of CFP-Cdc42p and Cdc12p-YFP. Plasmids p416(CFP-CDC42) and pRS315 containing CDC12-YFP under the control of the CDC12 promoter (32) were transformed into wild-type strain TRY11-7D, and transformants were grown to mid-log phase in SC−Leu−Ura−Met medium.