TABLE 1.
Yeast strains
| Strain | Genotype | Reference |
|---|---|---|
| C276 | MATa/MATα gal2/gal2 | 41 |
| DJTD2-16A | MATacdc42-1 his4 leu2 trp1 ura3 | 15 |
| DJD6-11 | MATa/MATα Δcdc42::TRP1/+ his3Δ200/+ his4/+ leu2/+ can1/+ lys2-801/lys2-801 trp1-Δ1/trp1-Δ101 ade2-101/+ ura3-52/ura3-52 | 25 |
| CSO-1B | MATacdc12-6 leu2 ura3 | B. Haarer |
| TRY11-7D | MATα leu2 ura3 trp1 his | 33 |
| W303 | MATa/MATα ade2-101/ade2-101 his3-11,5/his3-11,5 leu2-3,112/leu2-3,112 trp1-Δ1/trp1-Δ1 ura3-1/ura3-1 can1-100/can1-100 | R. Rothstein |
| TRY100 | MATa/MATα Δcdc42::TRP1/+ his3Δ200/+ his4/+ leu2/+ can1/+ lys2-801/lys2-801 trp1-Δ1/trp1-Δ101 ade2-101/+ ura3-52/ura3-52:pRS306(p42-GFP-CDC42):URA3 | This study |
| YEF2125 | MATa/MATα his3/his3 leu2/leu2 lys2/lys2 trp1/trp1 ura3/ura3 HOF1:HA::HIS3/HOF1:HA::HIS3 myo1::HIS3/myo1::HIS3 | 38 |
| YEF2126 | MATa/MATα his3/his3 leu2/leu2 lys2/lys2 trp1/trp1 ura3/ura3 HOF1:HA::HIS3/HOF1:HA::HIS3 bni1::HIS3/bnil::HIS3 | 38 |