TABLE 2.

Timing of GFP-Cdc42p localization during the cell cycle^a

Localization pattern	No. of cells observed	Timing of event (min)
		Avg	Range
Incipient bud site	19	11.4 ± 2.9	High, 20; low, 7.5
Mother-bud neck	18	9.6 ± 3.4	High, 16; low, 5
bni1Δ	6	24.8 ± 2.7	High, 29; low, 21

^aFor the incipient bud site, time was measured from GFP-Cdc42p's first clustering at the incipient bud site to the start of bud emergence in wild-type cells (TRY11-7D). Fluorescence at this time was captured approximately every 5 min. The timing of GFP-Cdc42p localization at the mother-bud neck region was measured from the first clustering of GFP-Cdc42p at the mother-bud neck region to the time it appeared as two distinct bands on mother and daughter cells in wild-type cells (TRY11-7D). Time was measured from the first clustering of GFP-Cdc42p at the mother-bud neck region to the time it appeared as two distinct bands on mother and daughter cells in the bni1Δ mutant (YEF5126).