FIG. 3.

Dose response for inhibition of parasite growth by compound 1. Dose-response curves indicating IC₅₀ values (see Table 2) for in vitro-grown parasites are shown for a representative experiment as follows: ▪, RHΔHXGPRT wild-type strain; ▴, transgenic strain expressing Tg-PKG_FLAG; ▾, transgenic Tg-PKG_FLAG T761M; and ⧫, transgenic Tg-PKG_FLAG T761Q. The metabolic activity of parasites growing in HFF cell monolayers was measured by determining [³H]uracil uptake. IC₅₀ values were determined from plots of samples run in duplicate by using curve-fitting software from GraphPad Prism (v.3).