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MFα pheromone promotes but is not essential for mating. The isogenic wild-type (JEC21), mfα1,2,3 (WSC18), and mfα1,2,3 plus MFα1 (WSC56) strains were coincubated with a MATa wild-type strain (JEC20) on V8 agar mating medium in room light for 2 days at 24°C. The edges of the mating mixtures were photographed at ×100 magnification.
