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. Author manuscript; available in PMC: 2025 Aug 6.
Published in final edited form as: Mol Cancer Res. 2025 Feb 6;23(2):128–142. doi: 10.1158/1541-7786.MCR-24-0624

Figure 1: LGR5+ cancer stem cell enrichment in KRAS mutant CRC cells exposed to peristalsis.

Figure 1:

(A) Representative LGR5 flow cytometry plots of HCT116 KRASMUT cells maintained in static controls or exposed to peristalsis in the bioreactor. (B) Box and whiskers plots summarizing flow analysis of KRAS mutant LGR5+ expression (%) after 24hr exposure to peristalsis bioreactor or maintenance in static controls. Significant increase in LGR5+ expression was noted in all cell types exposed to peristalsis compared to static controls (**p<0.01, ****p<0.0001, two-way ANOVA). (C) Representative LGR5 flow cytometry plots of RKO KRASWT cells maintained in static controls or exposed to peristalsis in the bioreactor. (D) Box and whiskers plots summarizing flow analysis of KRAS wild type LGR5+ expression (%) after 24hr exposure to peristalsis bioreactor or maintenance in static controls (*p<0.05, ns, two-way ANOVA). (E) Gene ontology pathway analysis using Weighted Gene Correlation Network Analysis in HCT116 KRASMUT and RKO KRASWT cells exposed to peristalsis. The top 9 GO terms from each population are displayed.