FIG. 3.

Bpt1p contributes to cadmium detoxification. Strains derived from ABC154 (A) and SM1058 (B) were tested for their capacity to tolerate CdSO₄. An aliquot of cells at 0.1 OD, and serial 10-fold dilutions thereof, were spotted onto plates containing 0, 10, or 30 μM CdSO₄ and incubated at 30°C for 3 days. The strains tested all harbor the insertless plasmid pSM217 to permit growth on SC-Ura drop-out media; they are (A) ABC154 (wild-type), ABC791 (bpt1Δ), ABC470 (ycf1Δ), and ABC794 (ycf1Δ bpt1Δ); and (B) SM1058 (wild-type), SM3794 (bpt1Δ), SM3851 (ycf1Δ), and SM3858 (ycf1Δ bpt1Δ).