Table 2.
Four Parallel Cultures of Four Independent FD Lymphoblast Lines[Note]
|
Expression Ratio in Week |
|||||
| Lymphoblast Culture andHarvest | A | B | C | D | Mean±SD |
| MIN9744: | |||||
| 1 | 2.15 | 1.81 | 200 | 181 | 1.94±0.16 |
| 2 | 2.18 | 2.09 | 2.14 | 1.97 | 2.09±0.09 |
| 3 | 2.93 | 2.94 | 3.00 | 2.89 | 2.94±0.05 |
| 4 | 4.21 | 3.50 | 3.67 | 3.78 | 3.79±0.30 |
| Mean | 2.87 | 2.59 | 2.70 | 2.61 | |
| Overall | 2.69±0.78 | ||||
| GUS14939: | |||||
| 1 | 1.46 | 1.47 | 1.45 | 1.56 | 1.49±0.05 |
| 2 | 2.75 | 271 | 2.90 | 2.80 | 2.79±0.08 |
| 3 | 2.19 | 1.90 | 2.00 | 2.00 | 2.02±0.12 |
| 4 | 2.67 | 2.22 | 2.81 | 2.18 | 2.47±0.32 |
| Mean | 2.27 | 2.08 | 2.29 | 2.14 | |
| Overall | 2.19±0.53 | ||||
| MIN9754: | |||||
| 1 | 3.14 | 2.63 | 3.26 | 3.07 | 3.03±0.27 |
| 2 | 4.10 | 3.72 | 3.56 | 2.39 | 3.89±0.30 |
| 3 | 3.80 | 3.27 | 3.67 | 2.48 | 3.54±0.24 |
| 4 | 3.21 | 2.39 | 3.41 | 2.42 | 2.63±0.39 |
| Mean | 3.56 | 3.00 | 3.24 | 3.27 | |
| Overall | 3.27±0.57 | ||||
| GUS28025: | |||||
| 1 | 2.96 | 1.59 | 1.86 | 1.79 | 2.05±0.62 |
| 2 | 2.49 | 1.94 | 2.50 | 2.53 | 2.37±0.28 |
| 3 | 3.56 | 3.38 | 3.39 | 3.44 | 3.44±0.08 |
| 4 | 2.42 | 1.97 | 2.11 | 2.58 | 2.27±0.28 |
| Mean | 2.86 | 2.22 | 2.47 | 2.59 | |
| Overall | 2.53±0.65 | ||||
Note.— Four independent FD lymphoblast cell lines were cultured over 4 wk to assess the consistency of WT:MU expression ratio in each individual cell line. Each cell line was split into four parallel cultures and assayed using densitometry as described.