Table 3.
Densitometric and Real-Time QPCR Analyses of Representative FD Postmortem Tissue and Cultured Cell Samples[Note]
| WT:MU Ratio |
||
| FD Postmortem Tissue | Densitometry | Real-Time QPCR |
| Temporal cortex | .35 | .35 |
| Medulla oblongata | .29 | .27 |
| Spinal cord | .25 | .43 |
| Sympathetic chain | .24 | .19 |
| Gasserian ganglia | .23 | .19 |
| Tonsil | 1.21 | 1.14 |
| Esophagus | .33 | .46 |
| Thyroid | .34 | .33 |
| Adrenal gland | .61 | .70 |
| Heart | .55 | .87 |
| Lung | .53 | .64 |
| Liver | .82 | 1.56 |
| Spleen | .94 | 1.00 |
| Skeletal muscle | .68 | .94 |
| Fibroblast | 1.33 | 1.01 |
| Lymphoblast | 3.53 | 3.44 |
Note.— The WT:MU IKBKAP ratio was calculated from data obtained by densitometry and real-time QPCR, using sample tissues represented in figure 2B. Results from the densitometric technique showed very high concordance with real-time QPCR values. The WT:MU data for both CNS and PNS tissues consistently produced a ratio <1.0, whereas cultured cells (lymphoblast and fibroblast) consistently yielded a ratio >1.0. QPCR values were expressed relative to the spleen as calibrator for both WT and MU levels, as described.