. 2003 Jan 15;72(3):691–703. doi: 10.1086/368295

Table A3.

PCR Primers Used for Mutation Detection in PRKCSH

	Oligonucleotide
Exon	Sense	Antisense	Annealing Temperature(°C)	Product Size(bp)
1^a	GTTCGCGGGCATTTTTCAGGAAC	GATCTCCCCAATCCTGGCCA	60	480
2^a	CGGGAAACTGAGTCAAAAGG	GTGAAGACACAGCGCATCTC	60	254
3^a	GGCACTGAGCAGTGTTCAATAA	ATGGGAGGACAGAGGTGGTA	60	244
4^a	GTGATGGGAGGGGTACTGTC	TCTGTGGATGGATGGGACAT	60	349
5^a	GTGATGGGAGGGGTACTGTC	TCTGTGGATGGATGGGACAT	60	349
6^a	TTGCAAGCCCACACTATGAG	GCAGACCTGGTGGATCCTAA	60	244
7^a	GCAGCATGATCAAAAACCTG	AGCTGGTCTCTTGCCTTCTG	60	274
8^a	AAGGAGGATCTGGCTGGTTT	GGGTGACAGAGGTGGCTTCT	60	205
9^a	CTCCCTAGAAGTCCCCAACC	AGGTCCTGAAGCAAGTTCCA	60	240
10^a	CAACCACTCCAGCCCCTGGT	CCAGGTGCCAGAACCAGAGG	61	390
11^a	GCAGGAGGGGCAGAGACACC	CCAAGATACTGGGGCTTGTG	60	500
12^a	GCAGGAGGGGCAGAGACACC	CCAAGATACTGGGGCTTGTG	60	500
13^a	CTGGGAGTCAAGGAGCAGTC	ATGAGGGTATGGGAGCACAC	59	227
14^a	TTCCCCAACCATCAGGAAACTG	AGACCCTCCTGTGTCTGTCG	60	260
15^a	TCCCCTGCCTTGCAGGCCT	GTTCCCCAAC CCATATGTCCC	60	320
16^a	TCCCCTGCCTTGCAGGCCT	GTTCCCCAAC CCATATGTCCC	60	320
17^a	GGTCCATCTTCCTCAGGGCC	CGAGCACCCG TCTGCCCATC	60	320
18^a	CTGGTCAACTCCTGGCCTCA	CACACCCCAGCAAAGCGAGG	60	480
10–13^b	HAF: ACAGACAGACGCCACCTCTT	HAR: TGGACTCCTCCATGTCCTTC	60	386
2–17^c	F2: GTGAAGACACAGCGCATCTC	17R: GGTCCATCTTCCTCAGGGCC	60	1741
10–17^c	HAF: ACAGACAGACGCCACCTCTT	Ex17R: GGTCCATCTTCCTCAGGGCC	60	893
1–4^d	F1: CTGCTGGACAAGAGGGGTGC	SCHR3: GACCCGGTTGGAGGGGATATA	60	402
1–8^d	F1: CTGCTGGACAAGAGGGGTGC	HBR: TGTCATCATCCAGCTCCTTG	60	800
1–11^d	F1: CTGCTGGACAAGAGGGGTGC	HCR: CTCCTCCTCCTCCTCTGTGG	60	1077
1–13^d	F1: CTGCTGGACAAGAGGGGTGC	HAR : TGGACTCCTCCATGTCCTTC	60	1290
2–4^d	F2: GTGAAGACACAGCGCATCTC	SCHR3: GACCCGGTTGGAGGGGATATA	60	313
2–8^d	F2: GTGAAGACACAGCGCATCTC	HBR: TGTCATCATCCAGCTCCTTG	60	711
2–11^d	F2: GTGAAGACACAGCGCATCTC	HCR: CTCCTCCTCCTCCTCTGTGG	60	988
2–13^d	F2: GTGAAGACACAGCGCATCTC	HAR : TGGACTCCTCCATGTCCTTC	60	1201
2–17^d	F2: GTGAAGACACAGCGCATCTC	17R: GGTCCATCTTCCTCAGGGCC	60	1741

PCR primers and condition for exon-by-exon amplification and mutation detection by DHPLC and direct sequencing.

RT-PCR for Northern probe.

RT-PCR for IVS16+1delGT mutation.

RT-PCR IVS2+6delTCC mutation.