Figure 2.

Intrachromosomal aberrations in chromosome 5 detected by use of the mBAND technique. Localization of the RSCP is shown in images captured using six different filters, specifically DAPI (A), FITC (B), Gold (C), Texas Red (D), Cy5 (E), and Aqua (F). Quantitative color ratio analysis of these different chromosome paints yields a “merged color” fluorescence intensity pattern (G) along the chromosome axis, indicating continuously changing color ratios. Finally, “pseudocolors” are assigned to sections of the chromosome with similar color ratios (H). Top, Intrachromosomal interarm aberration (pericentric inversion), as shown in figure 3b. Middle, Intrachromosomal intra-arm aberration (paracentric inversion), as shown in figure 3c. Bottom, Intrachromosomal intra-arm aberration (intra-arm deletion), as shown in figure 3d.