TABLE 2.
Plasmids used in this study
| Plasmida | Relevant markers |
|---|---|
| pCH1122b | CEN, URA3, ADE3 |
| pCH1023b | CEN, LEU2, ADE3 |
| pDK243c | CEN, LEU2; ade3-2p |
| pW35d | 2μm, URA3; SSD1/SRK1 |
| pGE36 | CEN, TRP1; DSN1-GFP |
| pGE55 | CEN, TRP1; NSL1-GFP |
| pGE74 | CEN, TRP1; DSN3-GFP |
| pGE81 | CEN, URA3; NSL1; pGE180 gap-repaired SnaBI-EcoNI in yeast strain YSLP1 |
| pGE82 | CEN, URA3; nsl1-6; pGE180 gap-repaired SnaBI-EcoNI in yeast strain YSL6 |
| pGE83 | CEN, URA3; nsl1-8; pGE180 gap-repaired SnaBI-EcoNI in yeast strain YSL8 |
| pGE84 | CEN, URA3; nsl1-68; pGE180 gap-repaired SnaBI-EcoNI in yeast strain YSL68 |
| pGE89 | CEN, URA3; DSN3(NSL2); pGE170 gap-repaired MluI-SpeI in yeast strain YSLP1 |
| pGE90 | CEN, URA3; dsn3-29/nsl2-29; pGE170 gap-repaired MluI-SpeI in yeast strain YSL29 |
| pGE99 | CEN, TRP1; NNF1 on a 1.4-kb XmnI-PvuII insert |
| pGE100 | CEN, TRP1; NNF1 on a 1.4-kb XmnI-PvuII insert and ADE3 on a 3.6-kb EagI-NheI insert |
| pGE101 | CEN, URA3; NNF1 on a 1.4-kb XmnI-PvuII insert |
| pGE102 | CEN, URA3; ade3-2p |
| pGE151 | 2μm, URA3; 1.4-kb HindIII subclone from YEp24-based library; contains DSN3(NSL2) |
| pGE164 | 2μm, URA3; 2.7-kb BsaAI subclone from YEp24-based library; contains DSN1 |
| pGE165 | CEN, URA3; 2.7-kb BsaAI subclone from YEp24-based library; contains DSN1 |
| pGE170 | CEN, URA3; 3.1-kb KpnI subclone from YEp24-based library; contains DSN3(NSL2) |
| pGE171 | CEN, URA3; 1.4-kb HindIII subclone from YEp24-based library; contains DSN3(NSL2) |
| pGE180 | CEN, URA3; 2.0-kb PvuII-NruI subclone from pCT3-based library; contains NSL1 |
| pGE190 | 2μm, URA3; 3.3-kb HindIII subclone from YEp24-based library; contains SLG1 |
a
Plasmids are from this study unless otherwise indicated.
b
From C. Holm.
c
From D. Koshland.
d
From K. Tatchell.