TABLE 4.
Tetrad data from DSN3 disruption
| Strain(s) (genotype) | % Spore viability | DSN3 (Leu− spores) | DSN3/dsn3::LEU2 (Leu+ spores)a | dsn3::LEU2 + pCEN/ URA3/DSN3 (Leu+ Ura+ spores)b |
|---|---|---|---|---|
| GEY160 (DSN3/DSN3) | 93 | 41 | NAc | NA |
| GEY210, GEY211, GEY212 (dsn3::LEU2/DSN3) | 40 | 35 | 4 | NA |
| GEY210, GEY211, GEY212 + pGE171 (dsn3::LEU2/DSN3 + pCEN/URA3/DSN3) | 60 | 43 | 5 | 19 |
a
Six out of the nine Leu+ spores were nonmating. All nine of the Leu+ spores still had a wild-type copy of DSN3 which was detected by PCR, as described in Materials and Methods.
b
All 19 of the Leu+ Ura+ spores were not able to lose pCEN/URA3/DSN3 on 5-FOA plates.
c
NA, not applicable.