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. 2003 Apr 14;72(5):1300–1307. doi: 10.1086/375116

Figure 2.

Figure 2

Detection of HADH2 388C→T mutation by RFLP analysis. The zygosity of the 388C→T mutation found in patients 2 and 3 (table 1) was studied using RFLP analysis. Genomic DNA was amplified using the primers 2284HADH2frw plus “M13 reverse”-tagged Ex3/4rev. PCR conditions were as described for the other genomic DNA amplifications. After amplification, the PCR products were incubated for 2 h at 37°C in the presence (+) or absence (−) of 10 units of BglII, and, afterward, the restriction products were separated on a 2% agarose gel.