FIG. 1.

Domain motifs in SP85 and expression constructs. (A) Domain model for SP85 based on amino acid sequence motifs and functional expression studies (39, 40). S.P., cleavable signal sequence. TXPP refers to the sequence of the tandem tetrapeptide repeats, which are likely to be O-glycosylated. Boxes numbered 1 to 5 are EGF-like C4C repeats. (B) Sequence organization of a general expression construct for myc-tagged secretory proteins in prespore cells (40) derived originally from pVEII. (C) Expression constructs examined in this study. N and C1C2 were previously described (40). N was expressed by ligating a cDNA for the N-domain into the BglII site of pVSB, yielding pVSBN. The NC1 and NC2 domain fusion constructs were created by ligating cDNAs encoding C1 or C2 into the BamHI site of pVSBN.