FIG. 4.
Calcofluor-positive spore numbers and cellulose production. For each strain, a plate of culminants originally seeded with 1.2 × 108 cells was suspended in 0.5% NP-40-KP to lyse nonspore cells, filtered to remove stalks, and centrifuged to recover sedimentable material. (A) Spores (or coats due to autogermination) were counted in a hemacytometer after labeling with Calcofluor White ST. After centrifugation, the pellet was extracted with hot urea-dithiothreitol and aliquots were degraded by trifluoroacetolysis or digestion with cellulase. Free Glc was assayed by high-pH anion-exchange chromatography. Data shown are averages of two independent determinations. (B) Aliquots were subjected to mild acid hydrolysis, and released Glc was assayed as described above. Data are given as fractions of total Glc assayed by trifluoroacetolysis (from panel A), which is plotted on a per Calcofluor-positive spore basis. Data are averaged from two independent trials.