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. 2002 Apr;1(2):293–303. doi: 10.1128/EC.1.2.293-303.2002

FIG. 4.

FIG. 4.

TSA treatment impairs IES excision. Southern hybridization analysis was used to monitor the effect of TSA treatment on the excision of the M, R, and PGM1 elements. Control DNA samples were isolated from vegetative CU427, CU428, and B2086 strains. Experimental DNA samples were isolated from the progeny of cells that were treated at 6 h, 8 h, 10 h, and 12 h after mixing with TSA (+TSA) and without TSA (−TSA) as indicated. Each sample represents a pool of about 100 progeny lines. Total DNA was digested with HindIII. The positions of the DNA fragments corresponding to the unrearranged forms (Mmic, Rmic, and PGM1mic) and the rearranged forms (Mmac, Rmac, and PGM1mac) are indicated by arrows. Among the different HindIII macronuclear fragments revealed by probe c, only the macronuclear fragment containing the PGM1 element is indicated by the arrow as PGM1mac. Conjugating cells were treated for 4 h (A) or continuously until 24 h after mixing (B). The same blot was successively hybridized with probes a, b, and c (Fig. 3) in both panels A and B.