FIG. 6.

TSA treatment does not affect chromosome breakage. Southern hybridization analysis was used to monitor chromosome breakage in sexual progeny of cells treated with TSA (+TSA) or without TSA (−TSA) for 4 h (panel A) or until 24 h (panel B). On the schematic representation of chromosome breakage, the 15-bp chromosome breakage site, Cbs, is drawn as a black box. The two macronuclear chromosomes generated after breakage are shown with hatched boxes designating telomeric repeats. All samples were digested with EcoRI. DNA samples in panel A are the same as in Fig. 4A, and DNA samples in panel B are the same as in Fig. 4B. Both blots were hybridized with probe 835a (56). The precise map and sequence of that genomic region have not been determined.