FIG. 2.

Amino acid alignment of M. grisea CBS1 (Mg) with S. cerevisiae CBS (Sc) (GenBank accession number AAC37401) and human CBS (Hs) (GenBank accession number A55760). Sequences were aligned by using the Clustal W program and Lasergene software (DNASTAR, Madison, Wis.). Gaps in the alignment are indicated by dashes. Asterisks indicate identical residues in all sequences. Colons indicate conservative substitutions. Dots indicate semiconserved substitutions. Lys119 in human CBS is involved in PLP binding and is conserved in M. grisea and S. cerevisiae (arrow). The Cys52 and His65 residues that axially coordinate the iron in the heme group in human CBS are doubly underlined. The CBS domains in the C termini of the sequences are underlined.