Figure  3.

ATLAS's reproducibility and robustness. a, High reproducibility. Genomic DNA samples (D1 and D2) from two unrelated individuals were digested, in duplicate, with AseI, and each digestion was ligated, in duplicate, to a compatible linker (L1–L4). Each of the purified ligation reactions (A1–A8) was amplified in duplicate, and the resultant primary amplifications (L1–L16) were radiolabeled in duplicate. The resultant ATLAS display patterns are shown for each of the 16 replicates. b, Robustness. Top, 5′ ATLAS performed, in duplicate, on 10 unrelated individuals (lanes 1–10). Controls (lanes 11–15) are the same as in figure 2. Bottom, Genomic DNAs (∼500 ng), used to generate the above 5′ ATLAS display patterns, resolved on a 0.8% agarose gel that contained ethidium bromide. Note the significant amount of degradation in the DNA sample from individual 3. MW = molecular weight markers; λ DNA = genomic DNA from bacteriophage lambda.