Table A.

Conditions Used for PCR

	Primer
Transcript	Forward	Reverse	PCR Conditions	Product size(bp)
NESP55	5′-GTCACTAATGGAGGACGCCGT-3′	5′-TTCGTAGCAGGCACGCACTCCTTCAACCTC-3	94°C × 3 min; 39 cycles of 94°C × 40 s, 62°C × 35 s, 72°C × 1 min; then 72°C × 5 min	400
XLαs	5′-GGATGCCTCCGCTGGTTTGAGCATCGGG-3′	5′-TTCGTAGCAGGCACGCACTCCTTCAACCTC-3′	94°C × 4 min; 39 cycles of 94°C × 40 s, 64°C × 30 s, 72°C × 1 min; then 72°C × 5 min	818
Exon 1A	5′-GGACACTCAGTCGCGTCGGCAC-3′	5′-TTCGTAGCAGGCACGCACTCCTTCAACCTC-3′	94°C × 3 min; 30 cycles of 94°C × 30 s, 72°C × 3 min; then 72°C × 5 min	510
Gsα	5′-ATGGGCTGCCTCGGGAACAGT-3′	5′-TTCGTAGCAGGCACGCACTCCTTCAACCTC-3′	94°C × 3 min; 35 cycles of 94°C × 1 min, 67°C × 35 s, 72°C × 40 sec; then 72°C × 5 min	482
Exon 1Abisulfite sequencing:
1st round	5′-TAGGGGTYGTTGTTATGGGT-3′	5′-AATAACAAAAATCTATTTACCCTCAAAC-3′	94°C × 4 min; 39 cycles of 94°C × 45 s, 62°C × 45 s; then 72°C × 6 min	…a
2nd round	5′-GGTTGYGTTAGGTGGTTGG-3′	5′-CTCCTTAATTTAACTCTTAAACAC-3′	94°C× 4 min; 39 cycles of 94°C × 45 s, 62°C × 45 s; then 72°C × 6 min	340

^aUse PCR product as template in second round.