Table 1.
Primers for Mutation Detection and PCR Amplification Conditions
| Exon | Forward Primer (5′→3′) | Reverse Primer (5′→3′) | Annealing Temperature | MgCl2 Concentration |
| 2 | GTTGTCAGCAGGAGAGCCTG | TGTCTCCAGGGTGGCAGGTC | 64°C | 3 mM |
| 3 | GCATTAAAAACAACTTGCTATC | ATGGAAATACCCTCATTAGCAG | 56°C | 2 mM |
| 4 | CCAATCAGCTGGACCCAATC | GTGATACTGACTTCCATGTCC | 62°C | 4 mM |
| 5a | CTGCTGATTGTTCTTTGTGTCG | ATTGTGTCACAGTTATGGAGTC | 61°C | 4 mM |
| 5b | ATAATTCATGTGACTACCAAGC | CCATTACACATTCAGTACACAC | 61°C | 4 mM |
| 5c | CCACCCACCATCAGAAAATTC | TCAGATGTCTAAAGAGGTCTG | 59°C | 3 mM |
| 5d | AGAAACCCTACAAATGCAGTG | CTCCAGTATGAATTCTCTGATG | 59°C | 2.25 mM |
| 5e | GGCCTTCATCCAGAAATCAC | CCACAGCCATTGCACTTATAG | 61°C | 4 mM |
| 5f | AAAGGCTTTTACTGACCAGTC | TGCATTCATAAGGCTTCTCTC | 59°C | 4 mM |
| 5g | AGAAATCGCACTTCATTGCGC | TCTGGATGAAAGCTTTCCCAC | 61°C | 4 mM |
| 5h | AGTCTCATACTGGAGAAAGAC | CTGCTATTAGATACCTGATGC | 59°C | 3 mM |