FIG. 2.

Adenylyl cyclase is required for mating in C. neoformans. (A) CAC1 wild-type, cac1 mutant, cac1 CAC1-reconstituted, gpa1 mutant, and gpa1 CAC1 strains were coincubated with the MATa strain JEC20 on V8 mating medium in the dark for 14 days at 30°C with and without cAMP (2.5 mM). (B) The edges of the mating mixtures were examined microscopically each day for mating hyphae and photographed after 7 days (×61).