FIG. 3.
Adenylyl cyclase mutants have defects in melanin production. CAC1 wild-type (WT), cac1 mutant, cac1 CAC1-reconstituted, gpa1 mutant, and gpa1 CAC1 strains were grown on Niger seed medium with (+) and without (−) cAMP (2.5 mM) at 37°C and photographed after 4 days. Strains that produce melanin are brown (gray on figure), whereas strains that produce less or no melanin are white.