FIG. 4.
Adenylyl cyclase mutants fail to induce capsule. (A) CAC1 wild-type (WT), cac1 mutant, cac1 CAC1-reconstituted, gpa1 mutant, and gpa1 CAC1 strains were incubated for 2 days under capsule-inducing conditions (DMEM–22 mM NaHCO3) with and without cAMP (20 mM). Capsule induction was qualitatively assessed with a standard India ink preparation and photographed (×61). (B) Capsule size was quantified by determining the packed-cell volume of normalized cell suspensions (109 cells/ml) for each sample. Data points represent the mean and standard error for triplicate samples.