FIG. 2.

Expression of ccg-9 is affected by exogenous stimuli that induce stress. Northern analysis of ccg-9 mRNA was performed following an environmental challenge. (A to C) Cultures were treated 24 h after transfer to darkness, a time corresponding to early subjective night (CT 15), and harvested at the indicated times after treatment. Genes affected by the different stimuli were used as internal positive controls, and 18S rRNA was used to verify equal loading of total RNA. (A) Osmotic stress induced by a change from 0 to 4% NaCl; (B) nitrogen starvation induced by a shift from 0.2 to 0% NH₄NO₃; (C) glucose starvation induced by a shift from 2 to 0% glucose; (D) heat shock induced by a shift to 50°C. For panel D, developing cultures were incubated for the indicated time at 25°C with or without 1 h at 50°C prior to harvest.