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. 2002 Feb;1(1):126–136. doi: 10.1128/EC.1.1.126-136.2002

FIG. 1.

FIG. 1.

Characterization of culB. (A) culB genomic locus. pBsr1 is a 4.1-kb plasmid with the selectable Bsr cassette that was inserted into the BamHI site of the culB gene (hatched rectangle). Genomic DNA from wild-type, culB− (clones 1 and 2) or culB− pkaR− (clone 3) cells was digested with BclI, separated by agarose gel electrophoresis, blotted, and probed with the XbaI-BamHI genomic DNA fragment (hatched bar) in a standard Southern analysis. Restriction enzyme sites: C, ClaI; X, XbaI: B, BamHI; Bc, BclI; D, DpnII. (B) Comparison of the predicted amino acid sequences of CulB, CulA, and human Cul1 (hCul1). The amino acid identities between CulB (AF144717), CulA (AF020287), and human Cul1 (U58087) are in black, and amino acid similarities are in highlighted in gray. (C) Expression of culB. RNA was extracted from cells at the indicated times after the initiation of development. RNase protection assays carried out on 10 μg of RNA from each sample revealed a single RNA species.