Fig. 2.

IHC staining of RCC tissue and derived primary cell cultures. The cells were detached, embedded in paraffin and stained for tissue- and tumor markers. a ccRCC expressed PAX8, vimentin (VIM), CD10 and CA9. b pRCC tissue and cell cultures expressed PAX8, VIM, CK7 and AMACR. c chRCC expressed PAX8, CK7 and c-kit (CD117); HE = hematoxylin–eosin; bar = 100 µm; The staining was assessed using a Leica DM500 microscope; ccRCC clear cell renal cell carcinoma, pRCC papillary renal cell carcinoma, chRCC chromophobe renal cell carcinoma