Fig. 4.

Treatment of RCC cell cultures with tyrosine kinase inhibitors and 2-DG. The primary cell cultures of eight ccRCC, two pRCC (here pRCC5 as example) and four chRCC were treated with tyrosine kinase inhibitors in combination with 2-DG (details see “Materials and methods”). The cell lines of ccRCC responded with a significant decrease of viability in the CellTiter-Glo assay. Additionally, the cells were treated with 2-DG alone. For ccRCC and chRCC, the median with the interquartile range is displayed. CcRCC clear cell renal cell carcinoma, pRCC papillary renal cell carcinoma, chRCC chromophobe renal cell carcinoma; CTRL untreated control, AXT axitinib, CBZ cabozantinib, PZP pazopanib, 2-DG 2-deoxy-d-glucose