Fig. 1.

Effect of single and combined treatment with AT101 and DMC on n-fold viability and n-fold amount of (%) dead cells in three different human primary GBM cell cultures. Cells were either treated with 5 µM AT101, 5 or 10 µM DMC as well as the combination of 5 or 10 µM DMC plus 5 µM AT101 for up to 6 days. a Cell viability was counted at day 0, 3 and 6, and numbers of vital cells were depicted as n-fold numbers of live cells compared to the internal stimulation control (DMSO) for days 3 and 6, respectively. b Cytotoxicity assay was performed at days 3 and 6 and dead cells were compared in relation to live cells of the respective day and stimulation (% were calculated) and shown as n-fold amount of (%) dead cells compared to the internal stimulation control DMSO for day 3 or 6, respectively. a + bn = 8 independent experiments (PC1), n = 4 independent experiments (PC2, 3). A two-way ANOVA followed by a Bonferroni’s multiple comparison test, comparing each column to all the other columns, of the shown data was performed to check significant differences. Only significances between the different stimulations and DMSO, and between the combined treatment to their respective single treatments are depicted (*p < 0.05, **p < 0.01, ***p < 0.001)