Fig. 2.

Selection of cell model and movement ability test. Screening results of mRNA and the protein expression of CEMIP among SCLC cell lines H446, H1688 and H209 and normal lung cell lines BEAS-2B by qRT-PCR and Western blot was presented in a and b respectively. p value ≤ 0.05 remains statistic significance. Efficiency of silencing and over-expressing CEMIP achieved by each LV-shRNA targets or plasmid transduction was examined both on mRNA (c and e) and protein level (d and f). In part g and h, transwell assays were carried out for comparing both migrated and invasive ability between H446 shCEMIP, H1688 OE and their negative control and parental cell lines. p value ≤ 0.05 remains statistic significance, ***p value ≤ 0.001, **p value ≤ 0.01, *p value ≤ 0.05