Fig. 1.

LRRK2 signaling pathway in the striatum and cortex is not affected by loss of PINK1 in vivo. (A) Schematic of methodology followed (B) Immunoblot of LRRK2 pathway component in mouse striatum and relative quantification of (C) pSer105/total Rab12, (D) phospho-threonine73/total Rab10, (E) pSer935/total LRRK2, and (F) PPM1H/Vinculin. Similarly in (G–K) analysis from the mouse cortex. Each lane was loaded with 40 μg of protein lysate from one mouse. In graphs, the black circle represents PINK1^WT while red square PINK1^KO animals. Box and whiskers plot, from min to max with the median line. Ordinary two-way ANOVA with Sidak’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.