Fig. 2.

Behavioral testing in 10.5-mo-old double-mutant PINK1 knockout/LRRK2 R1441C mutant mice does not suggest genetic interaction in vivo. (A) A battery of different tests was performed to assess motor function in PINK1 KO and LRRK2 [R1441C] knock-in mice (LRRK2^RC). (B) Weight at 10.5 mo. (C) Righting time from negative geotaxis test and (D) grip strength. (E) Measure of stride length during gait analysis and (F) time to turn from balance beam. (G) Time to fall from rotarod test. (H) Representative images of the microglial marker Iba1 and (I) quantification of the Iba1 positive cells. In violin plots, the black circle represents LRRK2^WT while red squares LRRK2^RC mice. Ordinary two-way ANOVA with Sidak’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. N = 15/16 mice per group. (Scale bar, 50 µm.)