Fig. 3.

LRRK2 signaling pathway is not affected by mitochondrial damage-induced activation of PINK1. PINK1 was activated with oligomycin/antimycin for 24 h, while LRRK2 was inhibited by 1 h and 30 min MLi2 treatment. (A) Representative immunoblot of PINK1 activation effect on LRRK2 pathway components in LRRK2^WT and LRRK2^RC MEFs. Quantification from three experimental replicates for pUb, pLRRK2, pRab10, pRab12, and PPM1H is presented in (B–F) respectively. Each lane was loaded with 30 μg of protein lysates from one well of a 6-well plate. In graphs, each dot represents one experimental replicate with two biological replicates. − denotes scramble siRNA while +PINK1 siRNA. Bar graphs show mean ± SEM from three independent experiments. Ordinary two-way ANOVA with Sidak’s multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.