Fig. 4.

Endogenous PPM1H is up-regulated following mitochondrial depolarization by a transcriptional mechanism. (A) Representative immunoblot from PPM1H^WT MEFs treated with oligomycin/antimycin A for 0, 4, 8, 16, and 24 h. (B) Quantification of PPM1H protein and (C) PPM1H mRNA from three independent experiments. (D) Representative immunoblot from PPM1H^WT and PPM1H^KO MEFs upon 24 h treatment with O/A, O/A + DRB, or O/A + CHX. (E) Quantification of PPM1H protein and (F) PPM1H mRNA from three independent experiments. Each lane was loaded with 40 μg of protein lysates. Graphs show mean ± SEM from three independent experiments. For B and C, ordinary one-way ANOVA with Dunnett’s multiple comparison test. For E and F, ordinary two-way ANOVA with Tukey’s multiple comparison test from three independent experiments. Empty circles denote dimethyl sulfoxide (DMSO) control while black circles represent O/A treated samples.