Fig. 1.

Inflammasome activation triggers cleavage of the Hippo kinases. (A) iBMDMs were challenged with the indicated Lp strains for 3 h. Levels of MST1, MST2, and vinculin (as internal controls) in cell lysate were determined by immunoblotting. MOI, multiplicity of infection. (B and C) Macrophages were challenged with the Lp strains for 3 h at MOI =10 for iBMDMs or MOI = 20 for RAW264.7 cells. (D) iBMDMs were primed with LPS for 3 h, followed by ATP stimulation for 3 h or nigericin for 4 h. Levels of MST1, MST2, GSDMD cleavage (as an indicator of NLRP3 activation) and vinculin (as internal controls) were detected by immunoblotting.