Fig. 3.
The Hippo kinases are critical for various death processes in macrophages upon ATP stimulation and Lp challenge. (A) LPS- or Pam3CSK4-primed iBMDMs were stimulated with ATP for 3 h. (B) Mst1/2−/− iBMDMs were transduced to express WT MST1 or the MST1D326A variant. The transduced iBMDMs were primed with LPS or Pam3CSK4 and stimulated with ATP for 3 h. (C) iBMDMs were challenged with the Lp strains at MOI = 20 for 3 h. (D) RAW264.7 macrophages were challenged with the Lp strains at MOI = 25 for 4.5 h. (E) iBMDMs were challenged with the Lp strains at MOI = 10 for 3 h. Levels and processing of indicated proteins in the cell lysates were analyzed by immunoblotting. Histone H2AX and vinculin served as internal controls.