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. 2025 Jan 30;122(5):e2418613122. doi: 10.1073/pnas.2418613122

Fig. 4.

Fig. 4.

Processing of the Hippo kinases is a downstream effect of the NLRC4 inflammasome. (A) WT or Nlrc4−/− RAW264.7 macrophages were challenged with the indicated Lp strains at MOI = 20 for 4.5 h. Levels and processing of the indicated proteins were determined by immunoblotting. (B) Cell lysates from HEK293T cells producing mouse NLRC4, NAIP5, caspase-1, and 6xmyc-tagged Lp flagellin FlaA were collected 24 h after transfection. Levels and processing of the indicated proteins were determined by immunoblotting. *nonspecific, cross-reacting band. An additional 42 kDa MST2-NT was observed in the cells with activated NAIP5/NLRC4 inflammasomes. (C) Mouse NAIP5/NLRC4 inflammasome was reconstituted in HEK293T cells with cotransfection of the flagellin genes encoded by different pathogens using transient transfection as described in B. (D) WT iBMDMs were pretreated with the caspase-1 inhibitor VX765 (100 μM) or caspase-8 inhibitor (200 μM) 1 h prior to challenge of the Lp02 strain (MOI = 20, 3 h). Levels and processing of the indicated proteins were analyzed by immunoblotting. Vinculin served as an internal control.