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. 2025 Jan 30;122(5):e2418613122. doi: 10.1073/pnas.2418613122

Fig. 5.

Fig. 5.

The Hippo kinases affect activation of caspases in wildtype macrophages in response to ATP and Lp challenge. (A) Presence or absence of the indicated proteins in the gene knockout iBMDMs was determined by immunoblotting. (B) Pam3CSK4-primed iBMDMs were treated with ATP for 5 h. Activation of caspase-1 and GSDMD cleavage in the cell lysates were analyzed by immunoblotting. (C) Pam3CSK4-primed WT and the gene knockout iBMDMs were stimulated with ATP for 5 h. Cell lysis was determined by the LDH release assay as described in Fig. 2A. Data were presented as mean ± SD of technical triplicates and representative of at least three independent experiments. *P < 0.05 compared to WT+ATP, #P < 0.05 compared to Gsdmd−/−+ATP. Student’s t test, two tailed; unpaired. (D) Cell lysates of the Pam3CSK4-primed iBMDMs after 5 h ATP stimulation were analyzed by immunoblotting. (E) Cell lysates of the iBMDMs challenged with Lp02 (MOI = 20, 3 h) were analyzed by immunoblotting. Vinculin served as an internal control.