Table 2.
ctDNA-based NGS assay detects multiple gene mutations in NSCLC
| Number | Mutation #1 | Mutation #2 | Mutation #3 | Mutation # 4 | Mutation # 5 |
|---|---|---|---|---|---|
| 10 | EGFR: p.E746_A750del | EGFR p.T790M | – | – | – |
| 4 | EGFR: p.L858R | EGFR p.T790M | – | – | – |
| 1 | EGFR: p.G719S | EGFR p.T790M | – | – | – |
| 1 | EGFR: p.G719S | EGFR p.L861Q | – | – | – |
| 3 | EGFR: p.E746_A750del | KRAS p.G13S | – | – | – |
| 2 | EGFR: p.L858R | KRAS p.G13S | – | – | – |
| 2 | EGFR: p.L858R | KRAS p.G12V | – | – | – |
| 1 | EGFR: p.L858R | KRAS p.G12A | – | – | – |
| 1 | EGFR: p.L858R | KRAS p.G12D | – | – | – |
| 1 | EGFR: p.L861Q | KRAS p.Q61R | – | – | – |
| 1 | EGFR: p.E746_A750del | PIK3CA: p.E545K | – | – | – |
| 1 | EGFR: p.E746_A750del | PIK3CA: p.E542K | – | – | – |
| 1 | EGFR: p.L858R | MET:p.Y1253D | – | – | – |
| 1 | BRAF: p.(G466A) | KRAS p.(G12D) | – | – | – |
| 1 | KRAS: p.G12V | KRAS: p.G13S | – | – | – |
| 1 | KRAS: p.G13S | ALK:p.L1196M | – | – | – |
| 1 | ALK:p.R1275fs | MAP2K1:p.P124Q | – | – | – |
| 9 | TP53 | EGFR | – | – | – |
| 10 | TP53 | KRAS | – | – | – |
| 2 | TP53 | NRAS | – | – | – |
| 3 | TP53 | PIK3CA | – | – | – |
| 1 | TP53 | ROS1 | – | – | – |
| 1 | TP53 | MAP2K1 | – | – | – |
| 1 | TP53 | ERBB2 | – | – | – |
| 3 | TP53 | TP53 | – | – | – |
| 1 | EGFR:p.T790M | KRAS:p.G13S | TP53:p.G245S | – | – |
| 1 | EGFR:p.746_750del | EGFR:p.C797F | PIK3CA:p.E545K | – | – |
| 1 | EGFR:p.L858R | EGFR:p.T790M | KRAS:p.G13S | – | – |
| 1 | EGFR:p.L858R | EGFR:p.C797F | KRAS:p.Q61fs | – | – |
| 1 | EGFR:p.746_750del | TP53:p.R282R | TP53:p.G245S | – | – |
| 1 | EGFR:p.746_750del | EGFR:p.T790M | NRAS:p.Q61fs | – | – |
| 1 | KRAS:p.G13S | TP53:p.R282W | TP53:p.R337H | – | – |
| 1 | BRAF:p.V600E | EGFR:p.(L858R) | KRAS:p.(G13S) | – | – |
| 1 | BRAF:p.V600E | KRAS:p.(G13S) | ALK:p.(R1275fs) | – | – |
| 1 | EGFR:p.G719S | BRAF:p.V600E | PIK3CA:p.H1047R | MAP2K1:p.Q56P | – |
| 1 | EGFR:p.L858R | EGFR:p.T790M | KRAS:p.G13S | TP53:p.V272Vfs | MAP2K1:p.P124L |
| 1 | EGFR:p.E746_A750del | KRAS:p.G13S | TP53:p.G245S | ALK:p.F1245fs | MET:p.H1112fs |
| 1 | EGFR:p.E746_A750del | KRAS:p.(G13S) | PIK3CA:p.E542K | ALK:p.(R1275fs) | – |