Figure 7.

In vitro OVA stimulation increases the intensity of transfer-mediated AHR. (A) Effect of in vitro OVA stimulation on transfer-mediated AHR. On day 18, spleen cells (5 × 10⁶ cells/ml) from OVA-sensitized mice were incubated with OVA (200 μg/ml) for 4 days in vitro. On day 22, recipients received these stimulated cells by intravenous injection (TRSTIM: 1 × 10⁶). AR was measured 4 days after the transfer (n = 5–10 per group). * p < 0.05 compared with PC₂₀₀Mch of TRSA (5 × 10⁷). # p < 0.05 compared with PC₂₀₀Mch of TROVA (1 × 10⁶). (B) BALF cytokine concentrations. Four days after the transfer, BAL was performed and then the centrifuged supernatant was assayed for IL-4, IL-5, and IFN-γ concentrations using ELISA, respectively (n = 5–10 per group). * p < 0.05 compared with the values of TRSA (5 × 10⁷). # p < 0.05 compared with the values of TROVA (1 × 10⁶).