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Identification of A. nidulans CaM binding proteins by overlay assay. A. nidulans crude protein extract was separated by polyacrylamide gel electrophoresis, transferred to an Immobilon-P membrane, and probed with either A. nidulans CaM, vertebrate CaM , S. cerevisiae CaM, or the Ca2+ binding mutant CaM-protein A fusion protein. The blots are identical exposures of 100 μg of crude protein extract probed in the presence of Ca2+ as described in Materials and Methods.
